Reutilization of Amino Acids in Protein Synthesis in HeLa Cells
نویسندگان
چکیده
منابع مشابه
Reutilization of amino acids in protein synthesis in HeLa cells.
On the assumption that all amino acids entering protein must pass through the intracellular pool of free amino acids, reutilization of leucine, lysine, arginine, and alanine was determined by pulse labeling of HeLa cell protein, followed by a chase in unlabeled medium and measurement of the specific activities of the intracellular free pool and protein amino acids. The half-lives of the amino a...
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The capacity of HeLa cell mitochondria, either isolated or in intact cells, to incorporate different labeled amino acids into proteins was investigated. Eight amino acids (alanine, arginine, aspartic acid, cysteine, glutamic acid, glutamine, glycine, and lysine), which include most of the charged polar ones, showed a very low amount, if any at all, of chloramphenicol-sensitive incorporation, re...
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Mitochondrial Protein Synthesis in Hela Cells
HeLa cell mitochondrial proteins have been shown to be the products of two separate protein-synthesizing systems; one, the general cellular mechanism, sensitive to inhibition by cycloheximide, the other, a specific mitochondrial system subject to inhibition by low concentrations of chloramphenicol (Galper, J. B., and J. E. Darnell. 1971. J. Mol. Biol 57:363). Preliminary data have suggested tha...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1971
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)61865-4